Capsaicin: The chemical compound responsible for the spiciness in chillies.
Wilbur Scoville's original test involved diluting a pepper extract in sugar water until the heat was no longer detectable by a panel of tasters. The more the extract had to be diluted, the higher its Scoville rating. While this method was a bit subjective, it was a great start for quantifying chilli heat.
These days, we have more precise ways to measure capsaicin concentration, like using High-Performance Liquid Chromatography (HPLC). This method provides more accurate and consistent results, but the Scoville scale is still used to express the heat levels of peppers.